The molar extinction coefficient is given as a constant and varies for each molecule. \(\epsilon\) is the molar extinction coefficient or molar absorptivity (or absorption coefficient),.\(A\) is the measure of absorbance (no units),.For this reason, Beer's Law can only be applied when there is a linear relationship. Figure 5: Transmittance (CC BY-4.0 Heesung Shim via LibreTexts)īeer-Lambert Law (also known as Beer's Law) states that there is a linear relationship between the absorbance and the concentration of a sample. The length \(l\) is used for Beer-Lambert Law described below. Figure 5 illustrates transmittance of light through a sample. With the amount of absorbance known from the above equation, you can determine the unknown concentration of the sample by using Beer-Lambert Law. Where absorbance stands for the amount of photons that is absorbed.
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